By Koki Horikoshi
The pioneering paintings of Dr. Koki Horikoshi at the discovery, learn and purposes of alkaliphilic micro organism are defined right here of their entirety. The isolation, distribution and taxonomy of alkaliphilic microorganisms in addition to their phone constitution and body structure are mentioned for a simple knowing of those entities. The molecular biology and genome sequencing of a few alkaliphilic bacterial traces also are presented.
Part II of the amount specializes in enzymes of alkaliphiles and their functions. those contain alkaline proteases, starch-degrading enzymes and various others. a few of these enzymes are at present in large advertisement use as laundry detergent ingredients and in wastewater treatment.
Alkaliphiles are a comparatively contemporary box of study that may doubtless offer avenues to quite a lot of extra discoveries and purposes for a brand new new release of employees in bioscience and technology.
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Extra info for Alkaliphiles: Genetic Properties and Applications of Enzymes
No. 9 Growth rate 54 46 44 44 46 Percent of sporulation 0 30-40 70 30-40 1 The final OD and the sporulation rate were measured after 47 h of incubation at 37 °C. (Reproduced from T. Kudo and K. Horikoshi, Agnc. Biol. , 43, 2613 (1979)) C. Germination Kudo and Horikoshi (Kudo 1983a, b) reported germination of alkaliphilic B. pseudofirmus No. 2b-2. 2 M NaCl solution spores of B. pseudofirmus No. 2b-2 germinated very well in the presence of both L-alanine and inosine, but germinated poorly in the presence of only L-alanine.
Recently, Sugiyama et al. (2004) analyzed the rotational characteristics of Na ^-driven flagellar motor in the presence and absence of coupling ion by the electrorotation method. The motor rotated spontaneously in the presence of Na^, and the rotation accelerated or decelerated following the direction of the applied external torque. The spontaneous motor rotation was inhibited by the removal of external Na ^; however, the motor could be forcibly rotated by relatively small external torque applied by the electrorotation apparatus.
Since 1968, Horikoshi and his coworkers have systematically studied alkaliphilic microorganisms (Horikoshi 1971a). They have paved the way in establishing a new microbiology, alkaliphilic microbiology, to use these microorganisms as microbial and genetic resources. 5 in Horikoshi-I medium. 0 and all indicated optimum pH value for growth above 8. Therefore, bacteria with pH optima for growth in excess of pH 8, usually pH 9 to 11, are defined as alka- 40 4 Physiology liphilic bacteria. This property is the most characteristic feature of these bacteria.