Download Biosurfactants: From Genes to Applications (Microbiology by Gloria Soberón-Chávez PDF

By Gloria Soberón-Chávez

Biosurfactants, tensio-active compounds produced by means of dwelling cells, at the moment are gaining expanding curiosity because of their power functions in lots of varied commercial components within which to this point virtually completely man made surfactants were used. Their distinct constructions and features are only turning out to be liked. furthermore, biosurfactants are thought of to be environmentally “friendly,” really non-toxic and biodegradable. This Microbiology Monographs quantity bargains with the newest advances within the box of microbial biosurfactants, resembling rhamnolipids, serrawettins, trehalolipids, mannosylerythritol lipids, sophorolipids, surfactin and different lipopeptides. every one bankruptcy reports the features of anyone biosurfactant together with the physicochemical homes, the chemical constructions, the position within the body structure of the manufacturing microbes, the biosynthetic pathways, the genetic legislation, and the capability biotechnological purposes.

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2001). Besides RhlR/C4-HSL quorum sensing, two additional cell-to-cell communication systems participate in the quorum sensing circuitry of P. aeruginosa and influence rhlAB transcription (Fig. 3). First, the rhl system is positively upregulated by another LuxR-type regulator called LasR, which is activated by its cognate AHL N-3-oxo-dodecanoyl-HSL (3-oxo-C12-HSL) (Latifi et al. 1996; Pearson et al. 1997). Second, besides the LuxR/AHL-type circuits, P. aeruginosa carries a distinct quorum sensing system composed of the transcriptional regulator MvfR (PqsR), which directs the biosynthesis of HAQs (De´ziel et al.

Most of the aforementioned methods result in the recovery of mixtures of different RLs congeners. Alternatively, chromatographic methods are usually the best solutions for separation of specific RL congeners in a pure form. These methods, however, work better after application of one of the extraction methods mentioned above. On the small scale, preparative TLC is a good choice (Monteiro et al. 2007; Sim et al. 1997); however, for large scale downstream processing, preparative column chromatography using silica gel is a better option (Burger et al.

Bredenbruch et al. (2005) suggested that the b-ketoacyl reductase RhlG plays a role, although an intact rhlG gene is not required for the production of HAQs. A more detailed description of the biosynthetic pathway of HAQs was recently described (Gross and Loper 2009). 2 Biosynthesis of Rhamnolipids-Rhamnose Moiety Rhamnose is a component of the cell wall lipopolysaccharide (LPS) core and of several O-antigen polysaccharides in a variety of gram-negative bacteria, including several strains of Pseudomonas (Burger et al.

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