By J. M. Bonga, Don J. Durzan (auth.), J. M. Bonga, Don J. Durzan (eds.)
2. 2. Plant fabrics 2. three. Pregrowth stipulations 2. four. Cryoprotectant remedy 2. five. Freezing 2. five. 1. gradual freezing 2. five. 2. swift freezing 2. five. three. Droplet freezing 2. 6. garage 2. 7. Thawing 2. eight. Viability trying out 2. nine. Post-thaw regrowth three. EXAMPLES OF CRYOPRESERVATION OF WOODY PLANT fabric four. power software OF CRYOPRESERVATION IN TREE development 17. NURSERY dealing with OF PROPAGULES - J. A. motive force, and 320 G. R. L. Suttle 1. advent 2. advertisement NURSERY wishes VS. LABORATORY perform three. SEASONALITY OF progress AND creation CYCLES four. MICROPROPAGATION recommendations four. 1. tendencies in advertisement micropropagation four. 1. 1. agreement micropropagation five. components AFFECTING SURVIVAL AND development five. 1. Hardening of propagules in vitro five. 2. Greenhouse considerationS------ five. three. box planting five. four. New techniques: Direct box rooting five. four. 1. Pretreatment in vitro five. four. 2. Root induction five. four. three. box placement 18. MYCORRHIZAE - R. okay. Dixon, and D. H. Marx 336 1. advent 2. function OF MYCORRHIZAE IN TREE development AND improvement three. creation and alertness OF ECTOMYCORRHIZAL FUNGUS INOCULUM three. 1. Bareroot inventory three. 2. Container-grown inventory four. box TRIALS WITH ECTOMYCORRHIZAL PLANTING inventory five. construction and alertness OF ENDOMYCORRHIZAL INOCULUM 6. box TRIALS WITH ENDOMYCORRHIZAL 7. study possibilities eight. precis 351 19. TISSUE tradition APPLICATIUN TO woodland PATHOLOGY AND PEST keep an eye on - A. M. Diner, and D. F. Karnosky 1. creation 2. HOST AND PATHOGEN: tradition AND problem 2. 1.
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Extra info for Cell and Tissue Culture in Forestry: Specific Principles and Methods: Growth and Developments
5 and to the particulate instability more of extensive investigation of its physiological role in plant cells growing in batch culture. An insight, albeit in a limited form, into the role of hexokinase is provided by continuous cultures of sycamore 39 cells. These cultures have an advantage over batch cultures in that they can be maintained in steady states in selected growth phases and at specific growth rates (103). In continuous cultures of ~ pseudoplatanus cells (28) the extractable activity of hexokinase remains low at various growth rates in comparison with the activities of other enzymes of carbohydrate oxidation.
Growth In most of plant cases cells with information on a the variety of carbon stability of these cultures and constancy of response over several passages is lacking. There have been relatively few reports of the establishment of suspension cultures from other forest trees studies of their physiology and biochemistry. Reinert (75) descr ibed a liquid coupled with An early report by culture initiated from dissociating callus of spruce (Picea glauca), but no quantitative growth data were provided.
This may make woody plants preferential subjects for research involving detailed studies of the anatomical/biochemical events associated with the protoplast developmental sequence. 4. Regeneration of shoots. The number o~ reports of shoot regeneration from protoplasts of hardwoods is limited to four (Table 1). Again one should note that the source tissue (nucellar callus) for the citrus example is highly juvenile and probably limited in application to citrus because of its specialized origin. It is interesting to note also that utilizing other source tissue for Citrus (such as leaves or cotyledons) has not produced successful results (7,8).