Download Cell Substrates: Their Use in the Production of Vaccines and by Harry M. Meyer Jr. M.D. (auth.), John C. Petricciani, Hope PDF

By Harry M. Meyer Jr. M.D. (auth.), John C. Petricciani, Hope E. Hopps, Paul J. Chapple (eds.)

This quantity stems from a symposium subsidized through the W. Alton Jones cellphone technological know-how middle, Lake Placid manhattan. the second one Annual W. Alton Jones cellphone technological know-how middle Symposium: cellphone Substrates and­ Their Use within the creation of Vaccines and different Biologicals was once held October 23-26, 1978. the guts is an operational unit of the Tissue tradition organization and gives, in collaboration with the Association's schooling Committee, a variety of academic and study actions. in past times two decades there were a number of nationwide and foreign meetings regarding telephone cultures used to provide organic items. these mostly handled the expertise and linked concerns that have been present on the time of the conferences. for instance, as human diploid cells have been constructed and proposed to be used in vaccine creation, a few conferences have been held to envision the professionals and cons of human diploid cells. a large number of facts used to be supplied at these meetings which shaped the foundation for the eventual attractiveness of that mobilephone procedure. every one assembly additional to the final base of data within the region of phone cultures and their program to the present and novel set of difficulties encountered. mostly, the contributors reaffirmed the elemental premises that have been formulated within the early days of polio virus vaccine construction concerning the standards for acceptability of cells while utilized in the manufacture of biologics meant for humans.

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Following such definition and leadership, the diploid cell stain concept was vigorously pursued in East and West Europe and approvals were granted by several National Health Authorities for use of vaccines produced in WI-38 cells. Opposition was continued in the USA, now based mostly on the concept put forth by Sabin (9-11) of the possible presence of human leukemia virus in diploid cell strains. No real basis was found to support this speculation. Meantime, storm clouds were gathering over live poliovirus vaccine produced in primary cell cultures (see 2).

The theory also provides a~interpretation~the variability in longevity of cell cultures of the same strain. This is a factor which should be taken into account when, for example, characterizing the longevity of new cell strains or performing diagnostic tests for diseases of premature aging. Reliable estimation of lifespan depends on the serial subculture of several independent populations. In addition, the bottleneck experiment shows that large changes in population size, even only temporary ones, can significantly alter culture lifespan.

This may be accomplished by working with the liberated products of the cells alone with care to avoid disrupting the cells themselves. This may not be possible in those situations in which the products of the cell membranes are to be harvested and separated. The second approach is to chop the DNA into bits by hydrolyzing it with DNA'se that has been shown to reduce the mean size to 4 nucleotides long (37,38). Dissociation from histones prior to enzyme treatment can be achieved, if necessary, by appropriate alteration in pH and ionic strength(39).

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