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By Harold C. Slavkin, Harold Slavkin

Extracellular Matrix impacts on Gene Expression

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S. and Pederson, T. (1973) Biochem. 72,2766-2773. ; and Kim, J. (1974) Arch. Bioch. 767,1-10. E. (1964) P. NAS US 52,93-100. H. 251-276, (L. , Amsterdam. E. (1963) P. NAS US 50,1026-1032. ; and Harbers, E. (1973) Bioc. Biop. A. 557,295-304. A. (1972) P. NAS US 69,3417-3421. ; and Stambaugh, R. (1974) Arch. Bioch. 767,11-19. Y. and Bonner, J. (1970) J. Mol. Biol. 45,469-487. H. L. (1962) J. Mol. Biol. 5,172-184. J. (1974) Science 755,817-824. 34 EXTRACELLULAR MATRIX INFLUENCES ON GENE EXPRESSION 24.

REFERENCES 1. Grobstein, C. (1954) Tissue interaction in the morphogenesis of mouse embryonic rudiments in vitro. In "Aspects of Synthesis and Order in Growth," pp. 233-256, Princeton Univ. Press, Princeton, New Jersey. 2. Grobstein, C. (1948) Optimum gonopodial morphogenesis in Platypoecilus maculatus with constant dosage of methyl testosterone. J. Exp. Zool. 109:215-237. 3. Weiss, P. (1947) The problem of specificity in growth and development. Yale J. Biol. and Med. 19:235-278. 4. Grobstein, C.

MATERIALS AND METHODS Murine Krebs II (KII) ascites tumor cells were propagated in the peritoneal cavity of adult Swiss-Webster mice according to Matthews and Körner (11). 14 M NaCl commencing on day 2. Krebs II cells were also propagated in vitro in suspension culture as described by Lapeyre and Bekhor (12). Chromatin was isolated by procedures developed by Bhorjee andPederson (13). During all operations, chromatin was maintained at 0-4°. Other procedures are as described by Bekhor et al. (8,14).

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