Download Ion-Selective Microelectrodes and Their Use in Excitable by Jiří Koryta (auth.), Eva Syková, Pavel Hník, Ladislav PDF

By Jiří Koryta (auth.), Eva Syková, Pavel Hník, Ladislav Vyklický (eds.)

The Symposium on "Ion Selective Microelectrodes and Their Use in Excitable Tissues" used to be held in Prague from July 8-11, 1980. It used to be prepared by means of the Institute of body structure of the Czechoslovak Academy of Sciences as a satellite tv for pc symposium of the XXVIII Interna­ tional Congress of Physiological Sciences in Budapest. Sixty parti­ cipants met for 3 days within the ancient surroundings of the Strahov Monastery. The casual and secure surroundings of the clinical classes, including the social programme made the assembly successful and helped to cement outdated friendships and to shape new ones. The organizers have been satisfied to welcome as contributors representatives from lots of the laboratories operating with ion-selective microelectrodes (ISMs) on the earth. Neurophysiological learn with liquid ion-exchanger ISMs within the Prague laboratory used to be begun as early as 1971 for the reason that considered one of us (P. H. ) had the potential of studying the strategy at once from Dr. J. L. Walker in Salt Lake urban. It was once because of his endurance and, later, his courtesy in offering us with the liquid ion-exchanger for potassium and silicone that we have been in a position to get a begin in what grew to become out to be, in a 12 months or so, very robust foreign festival. This quantity comprises the papers offered on the Symposium. it truly is divided in line with the particular periods with names of the chair­ males, who helped the organizers to make the Symposium a true success.

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53. , 1976, "Ion and Enzyme Electrodes in Biology and Medicine", M. C. Lubbers, LA. Silver and W. , Urban and Schwarzenberg, Munich, Berlin, Vienna. 54. , 1978, "Ion-sensitive intracellular microelectrodes", Academic Press, London, New York, San Francisco. 55. , 1980, Calcium selective microelectrodes are much improved by a new poly(vinylchloride}gelled sensor, Science, in press. 56. , 1971, Ion-specific liquid ion exchanger microelectrodes, Anal. , 43:89A. 57. , 1977, Intracellular ionic activity measurements in nerve and muscle, Physiological Reviews, 57:729.

DISCUSSION The results of the experiments in which ai(Cl) was measured in quiescent preparations indicate that chloride 1S not passively distributed in sheep cardiac Purkinje fibers. Although no estimate was made of intracellular interference by other anions, estimates made in sheep Purkinje (Vaughan-Jones, 1979) and cat papillary (Spitzer and Walker, 1980) indicate that 3 roM is the upper limit of interference in mammalian cardiac muscle. Even when 3 mM is subtracted from the values shown in Figure 1, ai(Cl) is not reduced to a value consistent with passive distribution.

Given the electrical transfer properties of the ISCM are optimally set, the response time also depends on the diffusion processes on the interfaces of the microelectrodes (see Koryta, this volume). This circumstance finds its practical expression in a different time constant of the concentration changes (Tc) as measured with different ion exchangers (Ca 2+, Cl-, K+). , present volume). , 40:559. ),332:l77. , 1973, The equilibrations time course of [K+]o in cat cortex, Exp. , 17:190. , 1976, Neutral carrier Ca 2+ -microe1ectrode, Chimia, 30:204.

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