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By J. Sokatch (Eds.)

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U. within a given culture was determined by streaking or dilution and spreading on XGal agar. (c) The morphogenetic capacity of the culture as an integral population was determined by spotting an aliquot (approximately 1 μΐ) on XGal agar. The reproducibility of the patterns produced by these multicellular inocula was often assessed by repeated spottings. Subclonal cultures were produced in many different experiments carried out over several months for various reasons. Each culture was stored at room temperature in a closed vial as a stationary suspension, and the properties of cultures sometimes changed and sometimes remained constant.

The leftmost plate contained spots of the bl232 culture (upper right), its 10-2 and 10~ 4 dilution subcultures (middle and left), and the bl2321-bl2325 subclonal cultures. The middle plate contained spots of the bll32 culture (upper right), its 10-2 and 10~ 4 dilution subcultures, and the bl 1321-bl 1325 subclonal cultures. The rightmost plate contained spots of the b 1332 culture (upper right), its 10~ 2 (upper left), 10~ 4 (second row right), and 10~ 6 (second row left) dilution subcultures, and the M3321-M3325 subclonal cultures.

The rightmost plate contained spots of the b 1332 culture (upper right), its 10~ 2 (upper left), 10~ 4 (second row right), and 10~ 6 (second row left) dilution subcultures, and the M3321-M3325 subclonal cultures. The four-digit cultures were 106 days old when diluted for subculturing and subcloning, and the plates were photographed after 14 days of incubation. 2 . Pseudomonas putida COLONIES 51 allowed to grow up again to its original density before spotting (Fig. 11). Since the original culture was much older when spotted than its dilution subcultures, the observed similarities showed that the same pattern was produced by young bacteria from a fresh subculture as by old cells from the starting culture.

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