By Shain-dow & Wu, Ray (editors) Kung
Volumes 1 and a couple of of Transgenic vegetation gather vital info on transgenic plants which has seemed scattered in lots of diversified guides. those volumes are an important milestone in plant/agricultural biology, advertise the sensible software of recombinant DNA expertise, and help in reworking the rural undefined.
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Extra info for Transgenic Plants, Volume 2: Present Status and Social and Economic Impacts
Natl. Acad. Sci. A. 70, 598-602. , Drummond, Η. , Merlo, D. , Montoya, A. , Gordon, M. , and Nester, E. W. (1977). Cell, 11, 263-271. XXX Introduction Chrispeels, Μ. J. and Sadava, D. (1977). In Plants, Food and People, W. H. Freeman, San Francis co, p. 192. Cocking, E. C. (1984). In Handbook of Plant Cell Culture (P. V. Ammirato, D. A. Evans, W. R. Sharp and Y. ) Vol. 3, 1-8. Cohen, S. , Chang, A. C. Y , Boyer, H. , and Hellings, R. B. (1973). Proc. Natl. Acad. Sci. A. 70:3240-3244. Danna, K.
In addition, 31 seeds derived from 5 primary transgenic plants were all resistant to hygromycin at 40 μg/ml. The lack of segregation of the integration pattern of the foreign DNA in these offspring indicated that the primary transformed plants were homo zygous, probably because the protoplasts used for plasmid uptake were isolated from an embryogenic microspore-derived culture. The authors concluded that further genetic analysis of additional transgenic plants from independent experi ments, and determination of the ploidy levels of the cell suspension before transformation, should clarify this point.
1990). This system has facilitated direct interaction with chimeric genes, coupled with stringent selection procedures, for the production of transgenic rice plants. In this chapter, we will highlight the successes achieved in rice, which clearly indicate that major advances in other cereals soon will be forthcoming. 1 D N A DELIVERY I N T O RICE PROTOPLASTS Gene transfer into plant cells can be assessed within several hours of the transfor mation process by assaying the activity of the gene product.